Basic Information:
Series_titleBrassinazole treatment of arf2 and wild-type dark-grown seedlings
Series_summaryKeywords: arf2 vs. Col, BRZ vs. mock
Series_overall_designTotal RNA was extracted from 4-day-old, etiolated Arabidopsis seedlings grown on 0.5 µM BRZ or mock treatments and used to probe ATH1 microarrays (Affymetrix), according to manufacturer’s protocols. There are three independent biological replicates for each genotype and treatment, except for arf2 mutants with BRZ where only two arrays passed quality control. We performed standard Affymetrix quality-control procedures using the BioConductor packages simpleaffy. Expression was normalized and estimated using the gcRMA package of BioConductor.
Series_typeExpression profiling by array
Series_sample_idGSM282693 GSM282694 GSM282695 GSM282696 GSM282697 GSM282698 GSM282699 GSM282700 GSM282701 GSM282702 GSM282703