Information about Samples:
Sample_title04h low CO2 + light replicate A04h normal CO2 + light replicate A04h low CO2 + dark replicate A04h low CO2 + light replicate B04h normal CO2 + light replicate B04h low CO2 + dark replicate B
Sample_geo_accessionGSM77175GSM77176GSM77177GSM77178GSM77179GSM77180
Sample_typeRNARNARNARNARNARNA
Sample_channel_count111111
Sample_source_name_ch1full rosettefull rosettefull rosettefull rosettefull rosettefull rosette
Sample_organism_ch1Arabidopsis thalianaArabidopsis thalianaArabidopsis thalianaArabidopsis thalianaArabidopsis thalianaArabidopsis thaliana
Sample_characteristics_ch1full rosette, without rootsfull rosette, without rootsfull rosette, without rootsfull rosette, without rootsfull rosette, without rootsfull rosette, without roots
Sample_molecule_ch1total RNAtotal RNAtotal RNAtotal RNAtotal RNAtotal RNA
Sample_extract_protocol_ch14 x 3 rosettes were harvested and directly frozen by liquid nitrogen, inside the growth chamber. The material of 15 rosettes was ground in liquid nitrogen.Total RNA was extracted from 250 mg fresh weight using 5 ml TriZol. Organic compounts were removed by 2 chloroform extractions. Precipitation was done with sodium chloride/sodium citrate/2-propanol over night at 4°C. Ethanol wash of RNA pellet was done twice with 75% Ethanol. Total RNA was dissolved in pure H2O.4 x 3 rosettes were harvested and directly frozen by liquid nitrogen, inside the growth chamber. The material of 15 rosettes was ground in liquid nitrogen.Total RNA was extracted from 250 mg fresh weight using 5 ml TriZol. Organic compounts were removed by 2 chloroform extractions. Precipitation was done with sodium chloride/sodium citrate/2-propanol over night at 4°C. Ethanol wash of RNA pellet was done twice with 75% Ethanol. Total RNA was dissolved in pure H2O.4 x 3 rosettes were harvested and directly frozen by liquid nitrogen, inside the growth chamber. The material of 15 rosettes was ground in liquid nitrogen.Total RNA was extracted from 250 mg fresh weight using 5 ml TriZol. Organic compounts were removed by 2 chloroform extractions. Precipitation was done with sodium chloride/sodium citrate/2-propanol over night at 4°C. Ethanol wash of RNA pellet was done twice with 75% Ethanol. Total RNA was dissolved in pure H2O.4 x 3 rosettes were harvested and directly frozen by liquid nitrogen, inside the growth chamber. The material of 15 rosettes was ground in liquid nitrogen.Total RNA was extracted from 250 mg fresh weight using 5 ml TriZol. Organic compounts were removed by 2 chloroform extractions. Precipitation was done with sodium chloride/sodium citrate/2-propanol over night at 4°C. Ethanol wash of RNA pellet was done twice with 75% Ethanol. Total RNA was dissolved in pure H2O.4 x 3 rosettes were harvested and directly frozen by liquid nitrogen, inside the growth chamber. The material of 15 rosettes was ground in liquid nitrogen.Total RNA was extracted from 250 mg fresh weight using 5 ml TriZol. Organic compounts were removed by 2 chloroform extractions. Precipitation was done with sodium chloride/sodium citrate/2-propanol over night at 4°C. Ethanol wash of RNA pellet was done twice with 75% Ethanol. Total RNA was dissolved in pure H2O.4 x 3 rosettes were harvested and directly frozen by liquid nitrogen, inside the growth chamber. The material of 15 rosettes was ground in liquid nitrogen.Total RNA was extracted from 250 mg fresh weight using 5 ml TriZol. Organic compounts were removed by 2 chloroform extractions. Precipitation was done with sodium chloride/sodium citrate/2-propanol over night at 4°C. Ethanol wash of RNA pellet was done twice with 75% Ethanol. Total RNA was dissolved in pure H2O.
Sample_label_ch1biotinbiotinbiotinbiotinbiotinbiotin
Sample_label_protocol_ch1Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
Sample_taxid_ch1370237023702370237023702
Sample_hyb_protocolstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix procedures
Sample_scan_protocolstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix proceduresstandard Affymetrix procedures
Sample_descriptionArabidopsis thaliana pgm (Col-0) mutant 5-6 week old rosettesArabidopsis thaliana pgm (Col-0) mutant 5-6 week old rosettesArabidopsis thaliana pgm (Col-0) mutant 5-6 week old rosettesArabidopsis thaliana pgm (Col-0) mutant 5-6 week old rosettesArabidopsis thaliana pgm (Col-0) mutant 5-6 week old rosettesArabidopsis thaliana pgm (Col-0) mutant 5-6 week old rosettes
Sample_platform_idGPL198GPL198GPL198GPL198GPL198GPL198