Basic Information:
Series_titleTranscriptome analysis of ARRESTED DEVELOPMENT 3 mutant.
Series_summaryAims: Comparison of transcriptome between mutant and wild-type plant.
Series_summaryBased on the temperature sensitive period of the mutant the gene likely acts during the earliest stages of the specification of the leaf primordium.
Series_summaryBackground: The ARRESTED DEVELOPMENT 3 mutation causes a temperature dependent loss of all spongy mesophyll and most palisade parenchyma in developing leaves. Although these leaves lack most internal tissues excepting vasculature they continue to expand away from the main axis of plant growth. Mature leaves have a small midrib and marginal regions that are large balloons of epidermis covering airspace. add3 is a temperature sensitive mutation whose most severe phenotypic response occurs at a restrictive temperature of 29 degrees C.
Series_summaryUsing BAC and TAC filters a recombinant population we have generated and available and newly developed molecular markers a high resolution (1000 chromosomes scored) physical genetic map has been completed to define the physical extent of the locus.
Series_summaryExperimentalia: Wild-type and add3 tissue will be grown at 29 degrees C until the first two true leaves have emerged. Two days after emergence the aerial portions of the plants will be collected and RNA will be isolated. This developmental stage will provide plants with primordia in various stages of development.
Series_summaryThe wild-type tissue will serve as a control for exposure of developing primordia to high temperature the add3 plants will provide the experimental transcriptome.
Series_summaryWe intend to use RNA isolation protocols provided by the former AFGC facility for isolation of RNA.
Series_summaryConclusion:ADD3 is the first mutant from our collection of temperature sensitive mutations affecting shoot, floral and root organogenesis to be studied at the transcriptome level.
Series_summaryOur work modeling the evolution of gene duplicates and genetic redundancy suggests that temperature conditional redundancy may be common in plants (Pickett and Meeks-Wagner Plant Cell 19957 1347-1356)(Force,Lynch,Pickett et al. Genetics 1999151 1531-1545).
Series_summaryAn exploration of the utility of transcriptomics to describe phenotypic impacts of potential conditionally redundant mutants is particularly timely. Many reverse genetics experiments in Arabidopsis result in mutants displaying small or no impacts on phenotype.
Series_summaryThe completion of the Arabidopsis genome sequence suggests that this experience may become common.
Series_summaryExperimenter name = F. Bryan Pickett
Series_summaryExperimenter institute = Loyola University of Chicago
Series_summaryExperimenter address = Dept. of Biology
Series_summaryExperimenter address = Loyola University of Chicago
Series_summaryExperimenter address = 6525 N. Sheridan Rd.
Series_summaryExperimenter address = Chicago, IL
Series_summaryExperimenter zip/postal_code = 60626
Series_summaryExperimenter country = USA
Series_summaryKeywords: genetic_modification_design
Series_overall_design4 samples were used in this experiment
Series_typeExpression profiling by array
Series_sample_idGSM133409 GSM133410 GSM133411 GSM133412